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Results suggest that random priming is the optimal method for expression analysis of long genes. This method of RT may be appropriate for alternatively spliced genes and for genes without unique probes in the 3’-region.

The Universal Linkage System (ULS™) is a platinum-based technology that allows direct labeling of proteins and nucleic acids with a large variety of labels and haptens, including many fluorophores, biotin, and dinitrophenol.

DeCyder™ MS Differential Analysis Software (DeCyder MS) is a new tool for visualization, detection, identification and label-free relative quantification of LC-MS and LC-MS/MS data.

We present here an online system to handle Affymetrix sample submission and tracking, job management and lab processing. Many current microarray database solutions tend to focus on post sample-processing analysis. GenomeLIMS allows recording of all sample processing steps, there are no expression data analysis functions.

Using a novel quantitative real-time PCR assay, specific to the mature product, the expression level of a selected group of miRNAs was measured across a set of 30 primary adult acute myeloid leukemia (AML) with a normal karyotype.

In the proteomics area with the increasing capacity of the mass spectrometers, sample preparation is becoming a limiting step for protein identification. Magnetic particle based technology provides a rapid and easy solution for automation of the sample preparation step.

In the proteomics area with the increasing capacity of the mass spectrometers, sample preparation is becoming a limiting step for protein identification. Magnetic particle based technology provides a rapid and easy solution for automation of the sample preparation step.

MICROWAVE Plasma generates radical density characteristic of 2000C at much lower effective temperature and can be highly ionized (Low pressure ECR mode) or highly neutral (high pressure mode).

In this study, using Protein Kinase A (PKA) as are presentative assay system, peptide phosphorylation reactions carried out in 384 PH filter plates showed dependence on enzyme and substrate concentrations, and on time. Kinase inhibition studies demonstrated IC50 and Z’ values equivalent to those obtained in 96-well plates. The higher throughput 384 well format offers a miniaturized version of the 96 well assay with equivalent sensitivity and reproducibility.

MolPAGE (Molecular Phenotyping to Accelerate Genomic Epidemiology) is an EU consortium with almost twenty collaborating universities and companies throughout Europe. One of the aims of MolPAGE is to find early onset biomarkersfor type 2 diabetes (T2DM) and cardio-vascular diseases(CVD).