Latest App Notes & Case Studies

CellometerTM Auto T4 is routinely used for counting cells used in immunology research. This application note provides some details on methods of using the CellometerTM system, and shows images and counting data to illustrate a typical application. Viability determination is based on trypan blue dye exclusion. Cell concentration is determined automatically based on total cell count and dilution factor. Cell size exclusion is used to count mature DC cells cultured from PBMC.

Plasmacytoid dendritic cells are principal players in the innate immune response to viruses. Although rare (less than 0.2% of circulating peripheral blood cells), they secrete over 3 pg of the potent antiviral cytokine IFN-a per cell in response to HSV, mainly via TLR-mediated signaling upon recognition of viral DNA motifs.

Molecular translocation of transcription factors from the cytoplasm to the nucleus is a pivotal event in many processes critical to cellular activation, diff erentiation and host defense. Existing methods used to measure nuclear translocation have significant limitations.

Cyanide is a well known acute toxin that prevents cellular respiration by irreversibly binding with the iron in cytochrome C oxidase. In addition, thiocyanate, which is metabolized from cyanide, interferes with iodine uptake by the thyroid gland, causing goiters and other long-term iodine deficiency diseases.

Compositional data of peptides and proteins can be determined using an amino acid analyser. If the identity of a protein is unknown, the compositional data of the protein can be used in searching protein databases.

Amino acids play an essential role in the nutritional composition of feedstuffs, however for many years diets were formulated to meet the requirements of crude protein rather than specific amino acids.

Using the Biochrom 30 Amino Acid Analyser, phenyalanine levels can be measured using a rapid, accurate programme for the diagnosis of PKU.

EPA Method 524.2, “Measurement of Purgeable Organic Compounds in Water by Capillary Column Gas Chromatography/Mass Spectrometry”, is a general-purpose method for the identification and simultaneous measurement of purgeable volatile organic compounds in surface water, groundwater, and drinking water.

The popularity and interest in biodiesel is significantly growing in many areas of the world and has become a commonly sought after alternative fuel source for use with diesel engines.

During multi-residue analyses, different groups of compounds are analyzed in a single run. Reducing time is one of the most important advantages of this approach. For environmental samples, different analyses usually have to be performed for each type of sample.